Journal: Oncotarget

Article Title: Landscape of RNAs in human lumbar disc degeneration

doi: 10.18632/oncotarget.11334

Figure Lengend Snippet: ( A – D ) delineate circRNA families binding with corresponding miRNAs. ( E and F ) indicate exceptional cases of multiple binding sites between circRNAs and miRNAs.

Article Snippet: Labeled cRNAs were hybridized onto the Arraystar Human circRNA Array (8 × 15 K, Arraystar).

Techniques: Binding Assay

Journal: Oncotarget

Article Title: Landscape of RNAs in human lumbar disc degeneration

doi: 10.18632/oncotarget.11334

Figure Lengend Snippet: ( A – B ) indicate miRNA-circRNA interacting machinery. ( C ) is the diagram of RNA transcription and splicing. ( D ) delineates the length scope of each type of RNAs.

Article Snippet: Labeled cRNAs were hybridized onto the Arraystar Human circRNA Array (8 × 15 K, Arraystar).

Techniques:

Journal: Oncotarget

Article Title: Landscape of RNAs in human lumbar disc degeneration

doi: 10.18632/oncotarget.11334

Figure Lengend Snippet: Extremes of RNA length

Article Snippet: Labeled cRNAs were hybridized onto the Arraystar Human circRNA Array (8 × 15 K, Arraystar).

Techniques:

Journal: Oncotarget

Article Title: Landscape of RNAs in human lumbar disc degeneration

doi: 10.18632/oncotarget.11334

Figure Lengend Snippet: ( A – B ) Represent the constituent ratio of circRNAs and differentially expressed circRNAs. ( C ) indicates the core interacting miRNA, circRNA, and mRNA in human lumbar discs.

Article Snippet: Labeled cRNAs were hybridized onto the Arraystar Human circRNA Array (8 × 15 K, Arraystar).

Techniques:

Journal: Cancer Management and Research

Article Title: Inhibition of microRNA-15b-5p Attenuates the Progression of Oral Squamous Cell Carcinoma via Modulating the PTPN4 /STAT3 Axis

doi: 10.2147/CMAR.S272498

Figure Lengend Snippet: miR-15b-5p is a potential prognostic biomarker indicating OSCC progression. ( A ) differentially expressed miRNAs between tumor tissues and normal tissues from OSCC patients screened out by a microarray analysis (n = 3); ( B ) miR-15b-5p expression in primary OSCC and normal tissues predicted on the TCGA database; ( C ) relevance between miR-15b-5p expression and the survival rate of OSCC patients predicted on the TCGA database; ( D ) miR-15b-5p expression in tumor and normal tissues from all OSCC patients determined by RT-qPCR (n = 37, * p < 0.05 according to paired t test); ( E ) miR-15b-5p content in metastatic and non-metastatic tumor tissues was determined by an RNA in situ hybridization assay; ( F ) relevance between miR-15b-5p expression and the survival of patients analyzed by the Kaplan-Meier analysis; high or low miR-15b-5p expression was determined according to the median value (2.53). Repetition = 3.

Article Snippet: The labeled RNA was hybridized onto the Arraystar Human miRNA array V4.0 (8 × 15K, Arraystar).

Techniques: Biomarker Discovery, Microarray, Expressing, Quantitative RT-PCR, RNA In Situ Hybridization

Journal: Molecular Therapy. Nucleic Acids

Article Title: hsa_circNFXL1_009 modulates apoptosis, proliferation, migration, and potassium channel activation in pulmonary hypertension

doi: 10.1016/j.omtn.2020.09.029

Figure Lengend Snippet: circRNA profile, the genome-wide landscape view, and hierarchical clustering of PAH-COPD patients and healthy controls (A) Volcano plot shows the different expression of circRNAs in two groups of subjects. Red/green dots indicate circRNAs expressed more/less than a 1.5-fold change in PAH-COPD patients. (B) Numbers of dysregulated circRNAs coming from different chromosomes. (C) Pie chart represents the percentage of dysregulated circRNAs coming from different chromosome regions. (D) Heatmap of 158 most significantly dysregulated circRNAs. (E) Heatmap of the top 40 dysregulated circRNAs (20 upregulated and 20 downregulated). Green/red represent a low/high expression level. PAH-COPD, pulmonary arterial hypertension due to chronic obstructive pulmonary disease; circRNA, circular RNA. n = 3 pairs of subjects. Comparisons of data were acquired by a Student’s t test.

Article Snippet: The circRNAs were transcribed into fluorescent cRNA and hybridized onto the Arraystar human circRNA array (8 ×15K, Arraystar) using a random priming method.

Techniques: Genome Wide, Expressing